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Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment ArchiMer
Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf
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